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Vimentin Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1595Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1595Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Vimentin Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Vimentin protein expression profile in cells. The kit can be used for measuring the relative amounts of Vimentin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Vimentin.
Synonyms
Vimentin; VIM
Gene Name
VIM
UniProt
P08670
Reactivity
Human, Mouse, Rat
Tissue Specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Molecular Weight
53652 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Vimentin
Subcellular Location
Cytoplasm.

UniProtKB · P08670

Vimentin

VIME_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08670
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VIM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B0YJC2, D3DRU4, Q15867, Q15868, Q15869, Q548L2, Q6LER9, Q8N850, Q96ML2, Q9NTM3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondPhosphoproteinS-nitrosylationUbl conjugation

Disease

CataractDisease variant

Cellular component

Cell membraneCell projectionCytoplasmCytoskeletonIntermediate filamentMembraneNucleus

Domain

Coiled coil

Biological process

Host-virus interaction