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Smad3 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1529Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1529Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Smad3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad3 protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad3.
Synonyms
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
Gene Name
SMAD3
UniProt
P84022
Reactivity
Human, Mouse, Rat
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Molecular Weight
48081 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Mothers against decapentaplegic homolog 3
Subcellular Location
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969) . Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081) . Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644) . MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245) . PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236) .

UniProtKB · P84022

SMAD family member 3

SMAD3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P84022
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMAD3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SMAD 3, hSMAD3
EC number
—
Processing
—
Secondary accessions
A8K4B6, B7Z4Z5, B7Z6M9, B7Z9Q2, F5H383, O09064, O09144, O14510, O35273, Q92940, Q93002, Q9GKR4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Disease

Aortic aneurysmDisease variant

Cellular component

CytoplasmNucleus

Molecular function

DNA-binding

Biological process

Host-virus interactionTranscriptionTranscription regulation

Ligand

Metal-bindingZinc

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