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Smad2 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1527Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1527Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Smad2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad2 protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad2.
Synonyms
Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2, MADR2
Gene Name
SMAD2
UniProt
Q15796
Reactivity
Human, Mouse, Rat
Tissue Specificity
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Molecular Weight
52306 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Mothers against decapentaplegic homolog 2
Subcellular Location
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696) . On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081) .

UniProtKB · Q15796

SMAD family member 2

SMAD2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15796
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMAD2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SMAD 2, hSMAD2
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmNucleus

Disease

Disease variantHeterotaxy

Molecular function

DNA-binding

Ligand

Metal-bindingZinc

Biological process

TranscriptionTranscription regulation

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