Rhodopsin Colorimetric Cell-Based ELISA Kit

• Description:

  The Rhodopsin Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Rhodopsin protein expression profile in cells. The kit can be used for measuring the relative amounts of Rhodopsin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Rhodopsin.

• Synonyms:

  Rhodopsin; Opsin-2; RHO; OPN2

• Gene Name:

  RHO

• UniProt:

  P08100

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Rod shaped photoreceptor cells which mediate vision in dim light.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071) . Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452) . Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425) . Subsequent receptor phosphorylation mediates displacement of the bound G- protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343) .

• Molecular Weight:

  38893 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Rhodopsin

• Subcellular Location:

  Membrane; Multi- pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia.