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Rhodopsin Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1505Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1505Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Rhodopsin Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Rhodopsin protein expression profile in cells. The kit can be used for measuring the relative amounts of Rhodopsin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Rhodopsin.
Synonyms
Rhodopsin; Opsin-2; RHO; OPN2
Gene Name
RHO
UniProt
P08100
Reactivity
Human, Mouse, Rat
Tissue Specificity
Rod shaped photoreceptor cells which mediate vision in dim light.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071) . Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452) . Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425) . Subsequent receptor phosphorylation mediates displacement of the bound G- protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343) .
Molecular Weight
38893 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Rhodopsin
Subcellular Location
Membrane; Multi- pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia.

UniProtKB · P08100

Rhodopsin

OPSD_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08100
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RHO
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q16414, Q2M249
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Cellular component

Cell projectionMembrane

Ligand

ChromophoreMetal-bindingZinc

Disease

Congenital stationary night blindnessDisease variantRetinitis pigmentosa

Molecular function

G protein-coupled receptorPhotoreceptor proteinReceptorRetinal proteinTransducer

Biological process

Sensory transductionVision

Domain

TransmembraneTransmembrane helix

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