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ILK Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1301Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1301Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The ILK Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ILK protein expression profile in cells. The kit can be used for measuring the relative amounts of ILK in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ILK.
Synonyms
ILK-1; ILK-2;59 kDa serine/threonine-protein kinase; P59ilk
Gene Name
ILK
UniProt
Q13418
Reactivity
Human, Mouse, Rat
Tissue Specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Receptor-proximal protein kinase regulating integrin- mediated signal transduction (PubMed:8538749, PubMed:9736715) . May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage- dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715) .
Molecular Weight
51419 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Integrin-linked protein kinase
Subcellular Location
Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cytoplasm, myofibril, sarcomere.

UniProtKB · Q13418

Scaffold protein ILK

ILK_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13418
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ILK
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B7Z1I0, B7Z418, D3DQU0, P57043, Q68DZ3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Domain

ANK repeatRepeat

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Cellular component

Cell junctionCell membraneCell projectionCytoplasmCytoskeletonMembraneNucleus

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