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HSP40 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1288Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1288Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The HSP40 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP40 protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP40 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP40.
Synonyms
DnaJ homolog subfamily B member 1; Heat shock 40 kDa protein 1; Heat shock protein 40; DnaJ protein homolog 1; HDJ-1; DNAJB1; DNAJ1; HDJ1; HSPF1
Gene Name
DNAJB1
UniProt
P25685
Reactivity
Human, Mouse, Rat
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401) . Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877) .
Molecular Weight
38044 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
DnaJ homolog subfamily B member 1
Subcellular Location
Cytoplasm. Nucleus. Nucleus, nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

UniProtKB · P25685

DnaJ homolog subfamily B member 1

DNJB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P25685
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DNAJB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B4DX52
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Chaperone

Cellular component

CytoplasmNucleus

PTM

Phosphoprotein

Biological process

Stress response

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