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HSP27 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1287Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1287Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The HSP27 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP27 protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP27 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP27.
Synonyms
28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27
Gene Name
HSPB1
UniProt
P04792
Reactivity
Human, Mouse, Rat
Tissue Specificity
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding- competent state (PubMed:10383393, PubMed:20178975) . Plays a role in stress resistance and actin organization (PubMed:19166925) . Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742) .
Molecular Weight
22783 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Heat shock protein beta-1
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub- nuclear structures known as SC35 speckles or nuclear splicing speckles.

UniProtKB · P04792

Heat shock protein beta-1

HSPB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04792
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HSPB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HspB1
EC number
—
Processing
—
Secondary accessions
B2R4N8, Q6FI47, Q96C20, Q96EI7, Q9UC31, Q9UC34, Q9UC35, Q9UC36
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoprotein

Molecular function

Chaperone

Disease

Charcot-Marie-Tooth diseaseDisease variantNeurodegenerationNeuropathy

Cellular component

CytoplasmCytoskeletonNucleus

Biological process

Host-virus interactionStress response

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