HSF1 Colorimetric Cell-Based ELISA Kit

• Description:

  The HSF1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSF1 protein expression profile in cells. The kit can be used for measuring the relative amounts of HSF1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSF1.

• Synonyms:

  Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSTF 1; HSTF1

• Gene Name:

  HSF1

• UniProt:

  Q00613

• Reactivity:

  Human, Mouse

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925) . In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218) . Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925) . Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218) . Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489) . Binds to chromatin at heat shock gene promoters (PubMed:25963659) . Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107) . Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK) -dependent manner (PubMed:14707147) . Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941) . Plays a role in the regulation of mitotic progression (PubMed:18794143) . Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage- dependent manner (PubMed:26359349) . Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925) .

• Molecular Weight:

  57260 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Heat shock factor protein 1

• Subcellular Location:

  Nucleus. Cytoplasm. Nucleus, nucleoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. The monomeric form is cytoplasmic in unstressed cells (PubMed:8455624, PubMed:26159920) . Predominantly nuclear protein in both unstressed and heat shocked cells (PubMed:10413683, PubMed:10359787) . Translocates in the nucleus upon heat shock (PubMed:8455624) . Nucleocytoplasmic shuttling protein (PubMed:26159920) . Colocalizes with IER5 in the nucleus (PubMed:27354066) . Colocalizes with BAG3 to the nucleus upon heat stress (PubMed:8455624, PubMed:26159920) . Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock (PubMed:11447121, PubMed:11514557, PubMed:10359787, PubMed:25963659, PubMed:10747973, PubMed:24581496, PubMed:19229036) . Colocalizes with SYMPK and SUMO1 in nSBs upon heat shock (PubMed:11447121, PubMed:12665592, PubMed:11514557, PubMed:14707147, PubMed:10359787) . Colocalizes with PRKACA/PKA in the nucleus and nSBs upon heat shock (PubMed:21085490) . Relocalizes from the nucleus to the cytoplasm during the attenuation and recovery phase period of the heat shock response (PubMed:26159920) . Translocates in the cytoplasm in a YWHAE- and XPO1/CRM1-dependent manner (PubMed:12917326) . Together with histone H2AX, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) (PubMed:26359349) . Colocalizes with calcium-responsive transactivator SS18L1 at kinetochore region on the mitotic chromosomes (PubMed:18794143) . Colocalizes with gamma tubulin at centrosome (PubMed:18794143) . Localizes at spindle pole in metaphase (PubMed:18794143) . Colocalizes with PLK1 at spindle poles during prometaphase (PubMed:18794143) .