DP-1 Colorimetric Cell-Based ELISA Kit

• Description:

  The DP-1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DP-1 protein expression profile in cells. The kit can be used for measuring the relative amounts of DP-1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DP-1.

• Synonyms:

  Transcription factor Dp-1; DRTF1-polypeptide 1; DRTF1; E2F dimerization partner 1; TFDP1; DP1

• Gene Name:

  TFDP1

• UniProt:

  Q14186

• Reactivity:

  Human

• Tissue Specificity:

  Highest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed:8405995, PubMed:7739537) . The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812) .

• Molecular Weight:

  45070 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Transcription factor Dp-1

• Subcellular Location:

  Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus and translocates into the nuclear compartment upon heterodimerization with E2F1.