Claudin 1 Colorimetric Cell-Based ELISA Kit

• Description:

  The Claudin 1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Claudin 1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Claudin 1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Claudin 1.

• Synonyms:

  CLD1; CLDN1; SEMP1; Senescence-associated epithelial membrane protein

• Gene Name:

  CLDN1

• UniProt:

  O95832

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391) .

• Molecular Weight:

  22744 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Claudin-1

• Subcellular Location:

  Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane. Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.