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B-RAF Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1065Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1065Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The B-RAF Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor B-RAF protein expression profile in cells. The kit can be used for measuring the relative amounts of B-RAF in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on B-RAF.
Synonyms
B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma viral oncogene homolog B1
Gene Name
BRAF
UniProt
P15056
Reactivity
Human, Mouse, Rat
Tissue Specificity
Brain and testis.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Molecular Weight
84437 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Serine/threonine-protein kinase B-raf
Subcellular Location
Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

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