AurA Colorimetric Cell-Based ELISA Kit

• Description:

  The AurA Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AurA protein expression profile in cells. The kit can be used for measuring the relative amounts of AurA in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AurA.

• Synonyms:

  AIK; ARK1; Aurora-A; Aurora-family kinase 1; Aurora-related kinase 1; Aurora/IPL1-related kinase 1; AYK1; Breast-tumor-amplified kinase; BTAK; IAK1; Ipl1- and aurora-related kinase 1; kinase AurA; Serine/threonine kinase 15; Serine/threonine kinase 6; Serine/threonine kinase Ayk1; STK15; STK6

• Gene Name:

  AURKAAIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6

• UniProt:

  O14965

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

• Molecular Weight:

  45809 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Aurora kinase A

• Subcellular Location:

  Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Detected at the neurite hillock in developing neurons (By similarity) . Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase (PubMed:9606188, PubMed:17229885, PubMed:21225229) . Colocalized with SIRT2 at centrosome (PubMed:22014574) . Moves to the midbody during both telophase and cytokinesis (PubMed:17726514) . Associates with both the pericentriolar material (PCM) and centrioles (PubMed:22014574) .