BRSK1 Colorimetric Cell-Based ELISA

• Description:

  The BRSK1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BRSK1 protein expression profile in cells. The kit can be used for measuring the relative amounts of BRSK1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BRSK1.

• Synonyms:

  Serine/threonine-protein kinase BRSK1;2.7.11.1; Brain-selective kinase 1;2.7.11.26; Brain-specific serine/threonine-protein kinase 1; BR serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1; SAD1 homolog; hSAD1; BRSK1; KIAA1811, SAD1, SADB

• Gene Name:

  BRSK1

• UniProt:

  Q8TDC3

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma- tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

• Molecular Weight:

  85087 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Serine/threonine-protein kinase BRSK1

• Subcellular Location:

  Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell junction, synapse. Localizes to synaptic vesicles in neurons (By similarity) . Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS- induced DNA damage.