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53BP1 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1007Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1007Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The 53BP1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor 53BP1 protein expression profile in cells. The kit can be used for measuring the relative amounts of 53BP1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 53BP1.
Synonyms
P53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1
Gene Name
TP53BP1
UniProt
Q12888
Reactivity
Human, Mouse, Rat
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136) . Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ) -mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306) . In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136) . Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600) . Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425) . Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity) . In contrast, it is not required for classic NHEJ and V (D) J recombination (By similarity) . Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112) .
Molecular Weight
213574 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
TP53-binding protein 1
Subcellular Location
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Localizes to the nucleus in absence of DNA damage (PubMed:28241136) . Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs) : recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600) . Associated with kinetochores during mitosis (By similarity) .

UniProtKB · Q12888

TP53-binding protein 1

TP53B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q12888
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TP53BP1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
53BP1, p53-binding protein 1, p53BP1
EC number
—
Processing
—
Secondary accessions
F8VY86, Q2M1Z7, Q4LE46, Q5FWZ3, Q7Z3U4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

ActivatorDNA-binding

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Cellular component

CentromereChromosomeKinetochoreNucleus

Biological process

DNA damageDNA repairHost-virus interactionTranscriptionTranscription regulation

PTM

Isopeptide bondMethylationPhosphoproteinUbl conjugation

Domain

Repeat

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