SDS Page Staining Solution
| Catalog number: | _x000D_B2010019A | _x000D_
| Lot number: | _x000D_Batch Dependent | _x000D_
| Expiration date: | _x000D_Batch Dependent | _x000D_
| Volume: | _x000D_100 mL | _x000D_
| Content | _x000D_Coomassie Blue in Methanol-acetic acid solution | _x000D_
| Staining Time | _x000D_20 to 60 min | _x000D_
| Appearance: | _x000D_Blue Solution | _x000D_
| Application: | _x000D_SDS PAGE gels staining | _x000D_
| Shelf-Life | _x000D_Two years from date of manufacture | _x000D_
| Storage: | _x000D_Room temperature | _x000D_
| Keywords: | _x000D_Coomassie blue buffer, gel staining buffer, SDS-PAGE staining reagent, coomassie blue staining, coomassie stain, SDS Page Staining Solution | _x000D_
| Grade | _x000D_Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. | _x000D_
| References | _x000D_1: Fernandez-Patron C, Hardy E, Sosa A, Seoane J, Castellanos L. Double staining_x000D_ of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl_x000D_ sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected_x000D_ proteins. Anal Biochem. 1995 Jan 1;224(1):263-9.2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining_x000D_ of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006_x000D_ Feb;67(2):358-62.3: Marshall T, Williams KM. Total protein determination in urine: elimination of_x000D_ a differential response between the coomassie blue and pyrogallol red protein_x000D_ dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8.4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium_x000D_ dodecylsulphate, dye concentration and paraprotein on coomassie blue dye-binding_x000D_ assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81.5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on_x000D_ Coomassie blue-stained two-dimensional cellulose acetate membranes. Anal_x000D_ Biochem. 1986 Nov 15;159(1):8-11.6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie_x000D_ brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-_x000D_ glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992_x000D_ Jun;24(3-4):265-74.7: Spector T. Refinement of the coomassie blue method of protein quantitation. A_x000D_ simple and linear spectrophotometric assay for less than or equal to 0.5 to 50_x000D_ microgram of protein. Anal Biochem. 1978 May;86(1):142-6. | _x000D_
| Related Products | _x000D_Biochemical Buffers and Solutions | _x000D_
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