Loading Buffer Reducing for SDS PAGE (2X)

CAT: 0952-B2010018Size: 10 mLDry Ice: NoHazardous: No
CAT#:0952-B2010018Size:10 mL
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Description
Reducing SDS PAGE Loading Buffer 2X _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Catalog number:B2010018
Lot number:Batch Dependent
Expiration date:Batch Dependent
Volume:10 mL
ContentSDS Page loading buffer 2X concentrate with DTT as reducing agent
pH6.8
Appearance:Blue Solution
Application:Sample loading buffer for SDS-PAGE electrophoresis under reducing conditions
Shelf-LifeTwo years from date of manufacture
Storage:Room temperature
Keywords:2X Laemmli Sample Buffer, SDS-PAGE Protein Loading Buffer 2X, Reducing Loading Buffer
GradeBiotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References1: Karlsson JO, Ostwald K, Kåbjörn C, Andersson M. A method for protein assay in_x000D_ Laemmli buffer. Anal Biochem. 1994 May 15;219(1):144-6.2: Sheen H. Quantitation of yeast total proteins in sodium dodecyl sulfate-_x000D_ polyacrylamide gel electrophoresis sample buffer for uniform loading. Anal_x000D_ Biochem. 2016 Apr 1;498:95-7.3: Amponsah A, Nayak B. Evaluation of the efficiency of three extraction_x000D_ conditions for the immunochemical detection of allergenic soy proteins in_x000D_ different food matrices. J Sci Food Agric. 2018 Apr;98(6):2378-2384.4: Cai X, Zheng Y, Speck NA. A Western Blotting Protocol for Small Numbers of_x000D_ Hematopoietic Stem Cells. J Vis Exp. 2018 Aug 22;(138):56855._x000D_ _x000D_ 5: Scully RR, Pressley TA, O'Neil RG. A site-directed antibody recognizes a_x000D_ component of the ouabain-binding domain of the alpha 1 subunit of rat_x000D_ Na+,K(+)-ATPase. Biochem Cell Biol. 1993 Nov-Dec;71(11-12):538-43._x000D_ _x000D_ 6: Guthrie CR, Murray LW, Kopchok GE, Rosenbaum D, White RA. Biochemical_x000D_ mechanisms of laser vascular tissue fusion. J Invest Surg. 1991;4(1):3-12._x000D_ _x000D_ 7: Rendić D, Wilson IB, Lubec G, Gutternigg M, Altmann F, Léonard R. Adaptation_x000D_ of the "in-gel release method" to N-glycome analysis of low-milligram amounts of_x000D_ material. Electrophoresis. 2007 Dec;28(23):4484-92._x000D_ _x000D_ 8: Wang X, Wang H, Wang D, Wang D, Han B, Tian W, Guo A. Thermal denaturation_x000D_ produced degenerative proteins and interfered with MS for proteins dissolved in_x000D_ lysis buffer in proteomic analysis. Electrophoresis. 2011 Feb;32(3-4):348-56.
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Related ProductsBiochemical Buffers and Solutions
Short Description
Catalog #: B2010018 (10 mL)
Weight
0.8
Length
2
Width
0.9
Height
0.9

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