HDAC3 Antibody

CAT: 0800-V9743SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V9743SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
O15379
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human HDAC3 protein was used as the immunogen for the HDAC3 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-HDAC3-2D4
Applications
ELISA, FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the HDAC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HDAC3 antibody is available for research use only.
Storage Conditions
Aliquot the HDAC3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the HDAC3 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using HDAC3 antibody (green, clone PCRP-HDAC3-2D4) and phalloidin (red) .

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