HDAC2 Antibody

• Description:

  Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

• Specifications:

  Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

• UniProt:

  Q92769

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 456-488 from the human protein were used as the immunogen for the HDAC2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IF, IHC-P

• Purity:

  SAS precipitation

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the HDAC2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This HDAC2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the HDAC2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the HDAC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  Western blot testing of human 293 cell lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.