HDAC4 Antibody

• Description :

  Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt :

  P56524

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 1049-1077 from the human protein was used as the immunogen for this HDAC4 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  IF, WB, IHC, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the HDAC4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This HDAC4 antibody is available for research use only.

• Storage Conditions :

  Aliquot the HDAC4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the HDAC4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Nucleus

• Image Legend :

  HDAC4 antibody western blot analysis in NCI-H292 lysate. Expected molecular weight: ~140 kDa (full length), ~95 kDa (truncated) .