Cyclin B1 Recombinant Antibody / CCNB1

• Description:

  It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

• UniProt:

  P14635

• Host:

  Rabbit

• Immunogen:

  Recombinant full-length human CCNB1 protein was used as the immunogen for the Cyclin B1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG κ

• Type:

  Recombinant

• Applications:

  ELISA

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Cyclin B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Cyclin B1 antibody is available for research use only.

• CAS Number:

  9000-83-3