CD3 delta Antibody

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3) . It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of g and e chains (ge), a heterodimer of d and e chains (de) and a homodimer of two z chains (zz) or a heterodimer of z and h chains (zh) . The z and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The g, e and d chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM) . In contrast, the z chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the z chain ITAMs has been solved.

9007-83-4

Immunohistochemistry (FFPE) : 1-2 µg/mL

P04234

Human

A recombinant fragment (within amino acids 1-171) of human CD3D protein was used as the immunogen for the CD3 delta antibody.

CD3D/8898

IHC-P

Protein A/G affinity

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Aliquot the CD3 delta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

This CD3 delta antibody is available for research use only.

Aliquot the CD3 delta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the CD3 delta antibody should be determined by the researcher.

Membrane