CD3 Antibody

• Description:

  CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P07766

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human T cell leukemia cells were used as immunogen for the CD3 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  B-B12

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  CD3 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  The CD3 antibody is available for research use only.

• Storage Conditions:

  CD3 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface and cytoplasmic

• Image Legend:

  Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone B-B12) and Reddot nuclear stain (red) .