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CD3 gamma Antibody / CD3g

CAT: 0800-RQ8022Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8022Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
T-cell surface glycoprotein CD3 gamma chain is a protein that in humans is encoded by the CD3G gene. The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P09693
Host
Rabbit
Reactivity
Human
Immunogen
E. coli-derived recombinant human protein (amino acids Q23-D165) was used as the immunogen for the CD3 gamma antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CD3 gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD3 gamma antibody is available for research use only.
Storage Conditions
After reconstitution, the CD3 gamma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CD3 gamma antibody should be determined by the researcher.
Location
Cell membrane
Image Legend
Immunofluorescent staining of FFPE human JK cells with CD3 gamma antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P09693

T-cell surface glycoprotein CD3 gamma chain

CD3G_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09693
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD3G
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q2HIZ6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Adaptive immunityImmunity

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

Molecular function

Receptor

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