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NCX1 Antibody / SLC8A1

CAT: 0800-RQ8309Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8309Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
In cardiac myocytes, Ca (2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca (2+) concentration during contraction is primarily due to release of Ca (2+) from intracellular stores. However, some Ca (2+) also enters the cell through the sarcolemma (plasma membrane) . During relaxation, Ca (2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca (2+) that entered across the sarcolemma must be extruded from the cell. The Na (+) -Ca (2+) exchanger is the primary mechanism by which the Ca (2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P32418
Host
Rabbit
Reactivity
Human
Immunogen
An E.coli-derived human recombinant protein (E301-Q686) was used as the immunogen for the NCX1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the NCX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NCX1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NCX1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NCX1 antibody should be determined by the researcher.
Location
Nuclear, plasma membrane
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with NCX1 antibody (green) and Phalloidin (red) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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