NELFE Antibody / Negative elongation factor E / RD

• Description:

  Negative elongation factor E (NELFE), also called RNA-binding protein RD, is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D) . The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P18615

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  E. coli-derived recombinant human protein (amino acids N262-D378) was used as the immunogen for the NELFE antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, IF, FACS, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the NELFE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This NELFE antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the NELFE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the NELFE antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Immunofluorescent staining of FFPE human A549 cells with NELFE antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.