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NELFE Antibody / Negative elongation factor E / RD

CAT: 0800-RQ8172Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8172Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Negative elongation factor E (NELFE), also called RNA-binding protein RD, is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D) . The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P18615
Host
Rabbit
Reactivity
Human
Immunogen
E. coli-derived recombinant human protein (amino acids N262-D378) was used as the immunogen for the NELFE antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the NELFE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NELFE antibody is available for research use only.
Storage Conditions
After reconstitution, the NELFE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NELFE antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human A549 cells with NELFE antibody (red) and Beta Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P18615

Negative elongation factor E

NELFE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P18615
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NELFE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
NELF-E
EC number
—
Processing
—
Secondary accessions
A2BE08, B4DUN1, B4DYX9, Q5JP74, Q5JP75, Q96F56, Q9NPK2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

ADP-ribosylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

ChromosomeNucleus

Domain

Coiled coilRepeat

Molecular function

RepressorRNA-binding

Biological process

TranscriptionTranscription regulation

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