Elongation Factor 2 Antibody / EEF2

CAT: 0800-RQ6172Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ6172Size:100 µg
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Description
Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P13639
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish
Immunogen
A human recombinant partial protein (amino acids M1-L858) was used as the immunogen for the Elongation Factor 2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Elongation Factor 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Elongation Factor 2 antibody is available for research use only.
Storage Conditions
After reconstitution, the Elongation Factor 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Elongation Factor 2 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
IHC staining of FFPE human breast cancer tissue with Elongation Factor 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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