Caspase 1 Antibody

CAT: 0800-RQ8114Size: 100 µgDry Ice: NoHazardous: No

1 / 1

CAT#:0800-RQ8114Size:100 µg

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Description

Caspase 1, apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. Caspase 1 was purified ICE from the cytosol of the THP. human monocytic cell line and found that the active protease was made up of 2 peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P29466

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids N132-H404) was used as the immunogen for the Caspase 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Caspase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Caspase 1 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) ThP-1 and 2) U937 cell lysate with Caspase 1 antibody. Predicted molecular weight ~45 kDa (full length) .

UniProtKB · P29466

Caspase-1

CASP1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P29466
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CASP1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: CASP-1
EC number: 3.4.22.36
Processing: Precursor
Secondary accessions: B5MDZ1, Q53EY6, Q6DMQ1, Q6GSS3, Q6PI75, Q9UCN3

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Cellular component

Cell membraneCytoplasmMembrane

Molecular function

HydrolaseProteaseThiol protease

PTM

Isopeptide bondPhosphoproteinUbl conjugationZymogen

Related Products

Catalog	Name
F43295-0.4ML	Caspase-6 Antibody
F52416-0.08ML	Caspase-3 Antibody
F42859-0.4ML	Caspase-10 Antibody
F48960-0.4ML	Caspase-3 Antibody
R36146-100UG	Caspase 12 Antibody
F42862-0.4ML	Caspase-2 Antibody
F42752-0.08ML	Caspase-6 Antibody
F50514-0.4ML	Caspase-3 Antibody
R32146	Caspase-7 Antibody
R35479-100UG	Caspase 6 Antibody

Alternative Products

Catalog	Name
abx007974-01	Caspase 12 (Caspase 12) Antibody
24290	Caspase Antibody
70R-49474	Caspase 9 antibody
20R-1428	Caspase 8 antibody
70R-11561	Caspase 1 antibody
70R-31080	Caspase 7 antibody
70R-13452	Caspase 14 antibody
70R-11740	Caspase 12 antibody
70R-31077	Caspase 3 antibody
70R-11565	Caspase 2 antibody