Dnmt3a Antibody

• Description:

  Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. [UniProt]

• Specifications:

  Western blot: 1:2000-1:4000

• UniProt:

  Q9Y6K1

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human Dnmt3a was used as the immunogen for the Dnmt3a antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1823CT281.43.36

• Applications:

  WB

• Purity:

  Protein G purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Dnmt3a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Dnmt3a antibody is available for research use only.

• Storage Conditions:

  Aliquot the Dnmt3a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Dnmt3a antibody at 1:2000: Lane 1) human T47D, 2) CCRF-CEM, 3) Jurkat and 4) HeLa cell lysate. Predicted molecular weight: 100-130 kDa.