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SMURF2 Antibody

CAT: 0800-F54130-0.05MLSize: 0.05 mLDry Ice: NoHazardous: No
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CAT#:0800-F54130-0.05MLSize:0.05 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:2000-1:4000
UniProt
Q9HAU4
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human SMURF2 was used as the immunogen for the SMURF2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1826CT865.51.28
Applications
WB
Purity
Protein G purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SMURF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMURF2 antibody is available for research use only.
Storage Conditions
Aliquot the SMURF2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the SMURF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of SMURF2 antibody at 1:2000: Lane 1) human MDA-MB-231 and 2) U-2OS cell lysate. Predicted molecular weight ~86 kDa.

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