RecombinantGM-CSF, Human (P.pastoris-expressed)

• Description:

  Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds[2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities[4]. Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells[5].Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

• Endotoxin:

  <1.0 EU/μg, determined by LAL method.

• Purity:

  > 95 % as analyzed by SDS-PAGE.

• Bioactivity:

  ED50 < 0.1ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of > 1.0x 10ˆ7 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100μg/ml.

• Molecular Weight:

  26-32 kDa, observed by SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against 10 mM PB, pH7.0.

• Host or Source:

  P. pastoris

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  MAPARS PSPSTQPWEH VNAIQEARRL LNLSRDTAAE MNETVEVISE MFDLQEPTCL QTRLELYKQG LRGSLTKLKG PLTMMASHYK QHCPPTPETS CATQIITFES FKENLKDFLL VIPFDCWEPV QE