RecombinantGMF-β, Human

• Description:

  Glia Maturation Factor beta (GMF-beta) is a 17 kDa brain specific protein that belongs to the ADF/cofilin superfamily. It is a neurotrophin that induces maturation of neurons and glial cells. Unlike other neurotrophins, GMF-β lacks a leader sequence and can be phosphorylated by protein kinase A and protein kinase C suggesting its role in signal transduction. GMF-β is a prominent mediator of inflammation in the central nervous system and can activate several inflammation-related genes such as tumor necrosis factor-α and interleukin-1β. Researchers have shown there are significantly higher levels of GMF-β protein in all the effected regions of Alzheimer’s disease (AD) brains suggesting an important role of GMF-β in AD pathogenesisRecombinant human Glia Maturation Factor beta (rhGMF-beta) produced in E.coli is a single non-glycosylated polypeptide chain containing 142 amino acids. rhGMF-beta has a molecular mass of 16.7kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% by SDS-PAGE and HPLC analyses.

• Bioactivity:

  Bioassay data are not available.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/mL.

• Molecular Weight:

  16.7 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant human Glia Maturation Factor beta (rhGMF-beta) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhGMF-beta remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  MSESLVVCD VAEDLVEKLR KFRFRKETNN AAIIMKIDKD KRLVVLDEEL EGISPDELKD ELPERQPRFI VYSYKYQHDD GRVSYPLCFI FSSPVGCKPE QQMMYAGSKN KLVQTAELTK VFEIRNTEDL TEEWLREKLG FFH