RecombinantFGF-9, Human

CAT: 0384-BK0049-01Size: 10 µgDry Ice: NoHazardous: No
CAT#:0384-BK0049-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibroblast Growth Factor-9 (FGF-9) is a heparin binding growth factor that belongs to the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development.Recombinant Human Fibroblast Growth Factor-9 (rhFGF-9) produced in E.coli is a single non-glycosylated polypeptide chain containing 208 amino acids. A fully biologically active molecule, rhFGF-9 has a molecular mass of 23.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 2.0 ng/ml , measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >5.0x 10ˆ5 units/mg.
Reconstitution
Reconstituted in ddH2O at 100 μg/ml.
Molecular Weight
23.4 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Human Fibroblast Growth Factor-9 (rhFGF-9) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-9 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MAPLGEVGN YFGVQDAVPF GNVPVLPVDS PVLLSDHLGQ SEAGGLPRGP AVTDLDHLKG ILRRRQLYCR TGFHLEIFPN GTIQGTRKDH SRFGILEFIS IAVGLVSIRG VDSGLYLGMN EKGELYGSEK LTQECVFREQ FEENWYNTYS SNLYKHVDTG RRYYVALNKD GTPREGTRTK RHQKFTHFLP RPVDPDKVPE LYKDILSQS

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