RecombinantFGF-6, Human

• Description:

  Fibroblast Growth Factor-6 (FGF-6) is a cytokine belonging to the heparin-binding FGF family, and is structurally related to other members of FGF family, particularly FGF-4. In vivo, FGF-6 exhibits an expression profile predominantly restricted tothe myogenic lineage, and it preferentially binds to two of the FGF receptors: FGFR1 and FGFR4. FGF-6 functions in muscle regeneration, myoblast proliferation and migration, and muscle differentiation in a dose-dependent manner. In vivo high concentration of recombinant FGF-6 up-regulates and down-regulates FGFR1 and FGFR4, respectively, as FGFR1 promotes the proliferation while FGFR4 promotes the differentiation in the muscle. Besides its dual function in muscle regeneration, FGF-6 may act as a regulator of bone metabolism as well.Recombinant human Fibroblast Growth Factor-6 (rhFGF-6) produced in E.coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhFGF-6 has a molecular mass of 18.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% as analyzed by SDS-PAGE and HPLC.

• Bioactivity:

  ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence 1 µg/mL heparin, corresponding to a specific activity of > 4x 10ˆ5 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/mL.

• Molecular Weight:

  18.8 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant human Fibroblast Growth Factor-6 (rhFGF-6) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-6 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  MGTRANNTL LDSRGWGTLL SRSRAGLAGE IAGVNWESGY LVGIKRQRRL YCNVGIGFHL QVLPDGRISG THEENPYSLL EISTVERGVV SLFGVRSALF VAMNSKGRLY ATPSFQEECK FRETLLPNNY NAYESDLYQG TYIALSKYGR VKRGSKVSPI MTVTHFLPRI