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MLF1 polyclonal antibody

CAT: 0384-BS5797-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS5797-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Myeloid leukemia factor 1 (MLF1) is a 268 amino acid protein expressed by a gene that is involved in translocations associated with acute myeloid leukemia. MLF1 is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. MLF1 induces p53-dependent cell cycle arrest in murine embryonic fibroblasts. MLF1 expression also inversely affects the endogenous level of COP1, a ubiquitin ligase for p53, inhibits Epo-induced cell cycle exit, and inhibits a rise in the cell cycle inhibitor p27. Polo-like kinase 1 (Plk1) phosphorylates MLF1 at its Thr78 site, which induces ubiquitination and degradation of MLF1 before the transition from metaphase to anaphase. Mutations of these phosphorylation sites stabilize MLF1 and inhibit mitotic progression. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1; MLF-1
Swiss Prot
P58340
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 194-239 of Human MLF1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 31 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MLF1 polyclonal antibody detects endogenous levels of MLF1 protein.
Applications Notes
Western blot (WB) analysis of MLF1 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
Host or Source
Rabbit

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