MASP2 polyclonal antibody

• Background :

  Mannose (or mannan) -binding lectin (MBL), also known as serum mannosebinding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation. In human, three types of MBL-associated serine proteases, MASP-1, MASP-2 and MASP-3, and a truncated form of MASP-2 (small MBL-associated protein; sMAP or MAp19) complex with MBL to activate the lectin pathway of the complement system. Activated MASPs subsequently cleave and activate downstream components of the complement pathway. MASP-3 is an alternatively spliced product from the MASP-1 gene and may function to inhibit MASP-2 by competing for MBL binding and inhibiting the activation of MBLassociated MASP-2.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Mannan-binding lectin serine protease 2; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2

• Swiss Prot :

  O00187

• Reactivity :

  Human, Mouse

• Immunogen :

  Synthetic peptide, corresponding to amino acids 268-313 of Human MASP2.

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:500~1:1000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 76 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  MASP2 polyclonal antibody detects endogenous levels of MASP2 protein.

• Applications Notes :

  Western blot (WB) analysis of MASP2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4