NBL1 polyclonal antibody

The DAN (differential screening-selected gene aberrative in neuroblastoma) protein family contains antagonists of bone morphogenetic protein (BMP) signaling that are expressed in the neural crest. All family members are secreted proteins that act as BMP antagonists in embryonic explants and are expressed in the proximal airway epithelium of the lung during embryonic development. This family includes the head-inducing factor Cerberus, the dorsaling factor gremlin and the tumor suppressor DAN. DAN, also known as zinc finger protein DAN, NO3, DAND1 or neuroblastoma suppressor of tumorigenicity 1 (NBL1), is a secreted protein containing one C-terminal cysteine knot-like (CTCK) domain. It is produced in small neurons of the dorsal root ganglion and its expression is activated by MATH-1. In addition to antagonizing BMP signaling, DAN also antagonizes the action of TGFβ. DAN is a possible tumor suppressor for human neuroblastoma; defects may result in or contribute to its progression. DAN may also be a neuromodulator in inflammatory pain.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Neuroblastoma suppressor of tumorigenicity 1; DAN domain family member 1; Protein N03; Zinc finger protein DAN; DAN; DAND1

P41271

Human, Mouse, Rat

Synthetic peptide, corresponding to amino acids 135-180 of Human NBL1.

Unconjugated

WB ICC

WB: 1:500~1:1000 ICC: 1:50~1:200

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 19 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

NBL1 polyclonal antibody detects endogenous levels of NBL1 protein.

Western blot (WB) analysis of NBL1 polyclonal antibody at 1:500 dilution Lane1:Jurkat cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lysate