BCAS2 polyclonal antibody

• Background:

  BCAS2 (breast carcinoma amplified sequence 2), also designated DAM1 (DNA amplified in mammary carcinoma 1 protein) or spliceosome-associated SPF 27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. BCAS2 is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligandindependent manner) with TRβ (thyroid hormone receptor β), ERα (estrogen receptor α), ERβ, PR (progesterone receptor), and PPARγ (Peroxisome proliferator-activated receptor γ) . BCAS2 functions as an ER coactivator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Pre-mRNA-splicing factor SPF27; Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27; DAM1

• Swiss Prot:

  O75934

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 180-225 of Human BCAS2.

• Conjugation:

  Unconjugated

• Applications:

  WB ICC IP

• Dilution:

  WB: 1:500~1:1000 ICC: 1:50~1:200 IP: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 26 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  BCAS2 polyclonal antibody detects endogenous levels of BCAS2 protein.

• Applications Notes:

  Western blot (WB) analysis of BCAS2 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Mouse heart tissue lysate Lane3:PC12 cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4