Matriptase (K21) polyclonal antibody

• Background:

  Matriptase (also known as MT-SP1, ST14, prostamin and epithin in mouse) is a tumor-associated type II transmembrane serine protease that is highly expressed in many human cancer-derived cell lines and is implicated in extracellular matrix re-modeling, tumor growth, and metastasis. Matriptase performs pleiotropic functions in the development of the epidermis, hair follicles, and cellular immune system. Sphingosine 1-phosphate (S1P, SPP), present in serum-derived lipoproteins, activates matriptase while matriptase activates both urokinase-type plasminogen activator and hepatocyte growth factor (HGF) . Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is a Kunitz-type serine protease inhibitor identified as a strong inhibitor of matriptase and HGF.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Suppressor of tumorigenicity 14 protein; Matriptase; Membrane-type serine protease 1; MT-SP1; Prostamin; Serine protease 14; Serine protease TADG-15; Tumor-associated differentially-expressed gene 15 protein; ST14; PRSS14; SNC19; TADG15

• Swiss Prot:

  Q9Y5Y6

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to the N-terminual of Human Matriptase.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF IP

• Dilution:

  WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 90 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Matriptase (K21) polyclonal antibody detects endogenous levels of Matriptase protein.

• Applications Notes:

  Western blot (WB) analysis of Matriptase (K21) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4