C/EBP-γ (E64) polyclonal antibody

• Background :

  The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  CCAAT/enhancer-binding protein gamma; C/EBP gamma; CEBPG; C/EBPγ; CEBPγ

• Swiss Prot :

  P53567

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 50-100 of Human C/EBP-γ.

• Conjugation :

  Unconjugated

• Applications :

  WB IHC IF

• Dilution :

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 22 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  C/EBP-γ (E64) detects endogenous levels of C/EBP-γ protein.

• Applications Notes :

  Western blot (WB) analysis of C/EBP-γ (E64) polyclonal antibody at 1:500 dilution Lane1:SGC-7901 whole cell lysate (40ug) Lane2:The testis tissue lysate of Mouse (30ug)

• Host or Source :

  Rabbit