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C/EBP-α (M15) polyclonal antibody

CAT: 0384-BS1384-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1384-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; C/EBP α; C/EBPα
Swiss Prot
P49715
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human C/EBP-α.
Conjugation
Unconjugated
Related Pathways
ESC Pluripotency and Differentiation Signaling Pathway
Applications
WB IHC
Dilution
WB 1:500 - 1:1000 IHC 1:50 - 1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 43 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
C/EBP-α (M15) polyclonal antibody detects endogenous levels of C/EBP-α protein.
Applications Notes
Western blot analysis of C/EBP alpha expression in mouse embryo (A), rat ovary (B), NIH3T3L1 (C), PC12 (D), Hela (E), Panc1 (F), SGC7901 (G) whole cell lysates.
Host or Source
Rabbit

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