C/EBP-γ polyclonal antibody

• Background:

  The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CCAAT/enhancer-binding protein gamma; C/EBP gamma; CEBPG; C/EBPγ; CEBPγ

• Swiss Prot:

  P53567

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length Human C/EBP-γ.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF

• Dilution:

  WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 16kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  C/EBP-γ polyclonal antibody detects endogenous levels of C/EBP-γ protein.

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using C/EBP-γ antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4