C/EBP-γ polyclonal antibody

CAT: 0384-BS8806-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS8806-01Size:50 µL
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Background
The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CCAAT/enhancer-binding protein gamma; C/EBP gamma; CEBPG; C/EBPγ; CEBPγ
Swiss Prot
P53567
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length Human C/EBP-γ.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 16kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
C/EBP-γ polyclonal antibody detects endogenous levels of C/EBP-γ protein.
Applications Notes
Western blot analysis of extracts of various cell lines, using C/EBP-γ antibody.
Host or Source
Rabbit

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