C/EBP ζ (D24) polyclonal antibody

• Background:

  C/EBP family members include C/EBP α, C/EBP β, C/EBP γ, C/EBP δ, C/EBP ε and C/EBP ζ, all of which are capable of forming homodimers or heterodimers with each other. C/EBP ζ, also known as CBF2, NOC1, CHOP10 (C/EBP homologous protein 10), GADD153 (growth arrest and DNA damage-inducible gene 153) or HSPCBF, is a ubiquitously expressed 1,054 amino acid protein that localizes to the nucleus and regulates transcription. More specifically, C/EBP ζ is believed to play a role regulating the expression of CRP (C-reactive protein) . Unlike most C/EBP dimers, C/EBP ζ-containing dimers do not bind the classical C/EBP-binding consensus sequence. Rather, C/EBP ζ-containing dimers must associate with RBP-Jκ (another transcription factor) in order to accomplish binding to the C/EBP site.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CBF2; CCAAT binding factor; CCAAT box binding transcription factor; CCAAT/enhancer binding protein zeta; Cebpz; HSPCBF; CBF

• Swiss Prot:

  Q03701

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to the N-terminus of Human C/EBP ζ.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~121 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  C/EBP ζ (D24) polyclonal antibody detects endogenous levels of C/EBP ζ protein.

• Applications Notes:

  Western blot (WB) analysis of C/EBP ζ (D24) pAb at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4