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Tsg 101 (E303) polyclonal antibody

CAT: 0384-BS2347-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS2347-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
It is now well accepted that the transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101
Swiss Prot
Q99816
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 268-320 of Human Tsg 101.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~45 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Tsg 101 (E303) polyclonal antibody detects endogenous levels of Tsg 101 protein.
Applications Notes
Western blot (WB) analysis of Tsg 101 (E303) polyclonal antibody in extracts from HEK293A cells.
Host or Source
Rabbit

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