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Cdc27 (K390) polyclonal antibody

CAT: 0384-BS2200-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS2200-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC; CDC27; ANAPC3; D0S1430E; D17S978E
Swiss Prot
P30260
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 360-410 of Human Cdc27.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 80, 92 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cdc27 (K390) polyclonal antibody detects endogenous levels of Cdc27 protein
Applications Notes
Western blot (WB) analysis of Cdc27 (K390) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug)
Host or Source
Rabbit

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