CDC23 polyclonal antibody

• Background:

  CDC23 shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Cell division cycle protein 23 homolog; Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8

• Swiss Prot:

  Q9UJX2

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 267-311 of Human CDC23.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 101% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 75 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CDC23 polyclonal antibody detects endogenous levels of CDC23 protein.

• Applications Notes:

  Western blot (WB) analysis of CDC23 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:RAW264.7 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4