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CDC27 polyclonal antibody

CAT: 0384-BS72040-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS72040-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CDC27; ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
Swiss Prot
P30260
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human CDC27 (NP_001107563.1) .
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
110kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Polyclonal Antibodies
Host or Source
Rabbit

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