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MDM2 (S160) polyclonal antibody

CAT: 0384-BS1447-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1447-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
P53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2
Swiss Prot
Q00987
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 130-180 of Human MDM2.
Conjugation
Unconjugated
Related Pathways
Examples of Crosstalk Between Post-translational Modifications, Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 55,90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MDM2 (S160) polyclonal antibody detects endogenous levels of MDM2 protein.
Applications Notes
Western blot (WB) analysis of MDM2 (S160) polyclonal antibody at 1:500 dillution Lane1:HeLa whole cell lysate (30μg) Lane2:PC12 whole cell lysate (30μg) Lane3:sp2/0 whole cell lysate (30μg)
Host or Source
Rabbit

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