MDM2 polyclonal antibody

• Background:

  E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  Double minute 2 protein; Hdm2; Oncoprotein Mdm2

• Swiss Prot:

  Q00987

• Reactivity:

  Human, Rat

• Immunogen:

  Synthetic peptide of human MDM2

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-P, ICC/IF

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 ICC: 1/100-1/200

• Purity:

  Affinity Purified

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 55 kDa; Observed MW: 90 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of MDM2 in SKOV-3 lysates using MDM2 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4