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Actin-α3/γ2 (E2) polyclonal antibody

CAT: 0384-BS1004-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1004-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. α actin expression is limited to various types of muscle, whereas β and γ are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Actin, gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin; ACTG2; ACTA3; ACTL3; ACTSG
Swiss Prot
P63267
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Actin-α3/γ2.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 45 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Actin-α3/γ2 (E2) polyclonal antibody detects endogenous levels of Actin-α3/γ2 protein.
Applications Notes
Western blot (WB) analysis of Actin-α3/γ2 (E2) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:SGC7901 whole cell lysate (40ug)
Host or Source
Rabbit

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