Cyclin E2 polyclonal antibody

• Background:

  The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1) . Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers.

• Structure Composition:

  Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Product Name Alternative:

  CCNE2; G1/S-specific cyclin-E2

• Swiss Prot:

  O96020

• Reactivity:

  Human, Mouse

• Immunogen:

  A synthesized peptide derived from human Cyclin E2

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-P, ICC/IF, FC

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

• Purity:

  Affinity Purified

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 47 kDa; Observed MW: 47 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of Cyclin E2 in HeLa lysates using Cyclin E2 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4